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i-screen KHV Kit Economical Testers
Waterproof Testers Waterproof Pocket Multi-Testers

Introduction:

The diagnostic kit i-screen offer the same sensitivity as Nested PCR which has been recognized so far the most sensitive viral detection tool. KHV detection can be performed without any professional training. By using i-screen, firsthand results can be obtained accurately in around three hours. The danger of KHV infection can be decreased to minimal and virus information is in control.

Specifications:

  • Packing size: 200 reactions.
  • Detection Limit: 10 copies/reaction.
  • Quantitative capability: 3 different levels of infection can be differentiated.
  • Sample throughput: for 40 samples, from sample preparation to final results require 3.5 to 4 hours.
  • Built-in internal control system: eliminate false negative results from failed extraction or reaction.
  • Quantified positive standard: monitor the sensitivity of detection.
  • Adaptable to the Uni-IQ RT-PCR profile, suitable for multi-viral diagnosis.

    Typical diagnostic results generated by
    i-screen KHV Detection and Prevention System.

  • Lane 1: KHV P(+) standard, 2000 copies/reaction
  • Lane 2: KHV P(+) standard, 200 copies/reaction
  • Lane 3: KHV P(+) standard, 20 copies/reaction
  • Lane 4: Negative control (yeast tRNA or ddH2O)
  • Lane 5: Sample with severe KHV infection
  • Lane 6: Sample with light KHV infection
  • Lane 7: KHV negative sample
  • Lane M: Molecular weight marker, 848 bp, 630 bp, 333 bp

    When to use:

    1. Quarantine before placing new koi into main pond.
    2. After sudden change of temperature.
    3. After long transportation.
    4. Suspicion of KHV.
    5. Protection and quarantine before competitions.

      Components:

      i-screen Starter Kit:

      Pipette Usage:

    		• Pipette in:
    1. Press plunger to first stop.
    2. Immerse the tip in the liquid.
    3. Allow the plunger to move up smoothly.
    4. Move pipette tip out of the tube.
    		Pipette out:
    1. Place pipette tip in the tube.
    2. Press plunger to first stop.
    3. Move pipette tip out of the tube.
    4. Allow the plunger to move up smoothly.

    Attention:

    1. Each time before sampling: clean both hands thoroughly with soap and dry them with tissue paper.
    2. Pre-heat the Reaction Oven beforehand at least 30 minutes.
    3. Strongly recommended allocating three separate spaces for a whole test process: Dissolving, Sampling, and Reacting.
    4. All components are for single test, reuse is not allowed. After detection, all tubes should be capped and discarded immediately.
    5. Please prepare bleach dilution in Reacting Space. (Dilute bleach in a bottle which be sealed with cap, eg. Mineral water bottle.) This is for disposing used Spotting Tips and Tube 2.
    6. Please mark clearly on different samples before tests begin by using marker pen or stickers. (Tube 0,1,2, and Detection Stick.)
    7. Swing each tube to bottom down the reagent every time before using.When using pipettes, press only to its First Stop. Please refer to "Pipette Usage" on the right.
    8. For first time users, please read carefully the "i-screen Instruction Manual" enclosed in Starter Kit. Should you require any help, please call the nearest distributor for further service and detail explanation.

    Dissolving

    Step 1
    Step 2
    1. Withdraw test kits from freezer equivalent to the number of samples planned to test. Allow it to defrost.
    2. Swing Tube 2-1 gently to bottom down the liquid.
    3. Apply Regular-Tip to Black Pipette and transfer the defrosted liquid from Tube 2-1 to Tube 2K once along the tube wall while transferring. Apply the same method to second 2K for dissolving.
    4. Do ignore the remaining liquid in Tube 2-1 and discard it.
    5. Remove Regular-Tip and discard it.
    1. Use Dropper to transfer the defrosted liquid all from 5-1 along the tube wall to Tube 5. Cap Tube 5 and swing gently.
    2. Move all test kits to sampling Space.
    Sampling

    *Please use Q-tip to scrape mucus for 5 times in red zone as indicated.
    Step 3
    1. Follow Sampling Instruction on the left for sample required.
    2. Cut off one side with mucus into Tube 0.
    3. Screw cap on and shake to form bubbles. Rest for 5 minutes.
    4. If samples are more than one, to avoid mistake, please mark samples in order on each cap of every test kit correspondingly.

    Step 4
    Step 5
    1. Swing Tube 1 to bottom down the liquid.
    2. Apply Filter-Tip to White Pipette and transfer upper liquid (no bubbles) from Tube 0 ONCE to Tube 1 by inserting its tip under liquid surface.
    3. Remove Filter-Tip and cap Tube 1 on. Flick it three times and swing it three times.
    4. To avoid contamination, please cap Tube 0 with remaining sample and discard it.
    1. Apply new Filter-Tip on White Pipette and transfer liquid from Tube 1 ONCE only by inserting its tip to 2K. Do not release the thumb until the tip remove from the liquid surface. Cap 2K on.
    2. Repeat previous step for another 2K. Cap 2K on.
    3. To avoid contamination, please cap Tube 1 with remaining liquid and discard it.
    Reacting

    Step 6
    Step 7
    1. Move all the remaining test kits to Reacting space.
    2. Place all Tube 2K on Tube Rack (Red) and move the rack into reaction Oven which has been pre-heated for at least 30 minutes.
    3. Adjust timer to 90 minutes. Activate the timer and start counting down.
    4. Note: In order to retain the temperature, avoid leaving the Reaction Oven door open for more than 10 seconds.
    5. Pour liquid bleach (around 3ml) in an empty bottle. Dilute with water 100 times to its total volume 300ml. Screw cap back on then it is ready to use.
    1. Withdraw the rack after 90 minutes.
    2. Prepare the Detection Stick and mark with sample number on every stick correspondingly.
    3. Insert Spotting Tip under liquid surface of Tube 2K and rest for 1 second. Later, touch the detection window of Detection Stick for 1 second. Immediately discard Spotting Tip and Tube 2K (cap on) into the bottle of bleach dilution.
    4. Repeat previous step for another Tube 2K with second Spotting Tip. Do not overlap two spotting areas.
    5. Screw the Detection Stick onto Tube 3 tightly. Repeat step 3 to 5 until all tests are completed.

    Step 8
    Step 9
    1. Place all Tube 3 on Tube Rack. Turn it over for 5 times then place them into Reaction Oven for 5 minutes.
    2. After 5 minutes, transfer and screw Detection Stick onto Tube 4. Submerge for 10 seconds under room temperature.
    1. Transfer and screw Detection Stick onto Tube 5. Place all Tube 5 on Tube Rack and turn it over for 5 times. Screw caps back on Tube 3 and 4 and discard them immediately.
    2. Put Tube Rack in Reaction Oven for 5 minutes.
    3. Later, transfer and screw the Detection Stick onto Tube 6. Submerge for 10 seconds under room temperature.
    4. Cap Tube 5 and discard it.

    Step 10
    1. Transfer and screw the Detection stick onto Tube 7. Place all Tube 7 back on Tube Rack and turn it over for 5 times. Put Tube rack in Reaction Oven for 10 minutes.
    2. Retain Tube 6 with cap on.
    3. After 10 minutes, return the Detection Stick to Tube 6 and submerge for 10 seconds under room temperature.
    4. Take out Detection Stick and lay it horizontally on Tube rack. Allow the detection window facing up and drying in Reacting Oven for 10 minutes.
    5. Withdraw Detection Stick for result interpretation.
    Result interpretation

    After detection, it is strongly suggested to discard Detection Sticks within 24 hours. Do not bring Detection Sticks out of reacting Area.

    Attention

    Due to special characteristic of KHV, positive result might not be shown during latent phase. The actual meaning of no KHV infection can be also interpreted as in latent phase.

    copyright © 2006 INTEROCEAN INDUSTRIES CO., LTD. All rights reserved.